This study investigates the positive and negative effects of online teaching on EFL students’ learning motivation. There are 26 English majors in a Chinese university participant in this study. Data were collected from multiple sources: semi-structured interview and direct observation. The result shows that in the course aspect, it has a positive effect. In the teacher aspect, it has both negative and positive effects. But in the learning group aspect, it has a negative effect, because of losing of students’ interaction.