The purpose of this paper is to analyze the artistic vitality in the late Ming Dynasty from three different perspectives: social function, collector group and collector noumenon. Art in the late Ming Dynasty is in a vigorous development trend. Through a series of collection activities of three representative collectors in Jiangnan area Xiang Yuanbian, Dong Qichang and Li Rihua, the vivid picture of art collection is connected, the historical situation of art creation in the late Ming Dynasty is reproduced, and the influence of collectors in Jiangnan on art vitality in the late Ming Dynasty is elaborated.