Keywords
Submitted : 2022-09-11
Accepted : 2022-09-26
Published : 2022-10-11
Abstract
In chemiluminescence reactions (CL), the electron jumps to a higher energy level when the fluorescent dye is excited. Dye in an excited state emits light by electrons jumping back to the ground state. In this review, indirect CL was studied with the main focus on the curve of CL intensity due to different concentrations of Rhodamine B (RhB) in Bis (2,4,5-trichloro-6-CarboPentoxyPhenyl) Oxalate (CPPO) solution with hydrogen peroxide (PO-CL). Because the light produced after the excitation of the dye by CPPO-CL is visible, the experiments were analyzed directly using a camera and programming. It was observed experimentally that the CL intensity would increase and then decrease with an increasing concentration of RhB. The resulting data are partially consistent with a Gaussian fit, but after excitation, the trend is more likely to fit consistent with that of logarithmic functions. As the CL intensity was reduced at an RhB concentration of 0.05g/20ml, there is a possibility that the quenching effect exists in the reaction of a high RhB concentration with CPPO. The difference in emission wavelengths and the time to reach maximum brightness are also discussed in this report.
References
Roda A, (eds) 2011, Chemiluminescence and Bioluminescence: Past, Present and Future, Cambridge, Royal Society of Chemistry, London.
Yan Y, Shi P, Song W et al., 2019, Chemiluminescence and Bioluminescence imaging for Biosensing and Therapy: In Vitro and In Vivo perspectives. Theranostics, 4(9): 4047–4065.
Fisher J, Sørensen OE, Abu-Humaidan AHA, 2020, Using Chemiluminescence Imaging of Cells (CLIC) for Relative Protein Quantification. Scientific Reports, 10(1): 1–11.
Wang Z, Huang J, Yu B, et al., 2021, Chemiluminescence: From Mechanism to Applications in Biological Imaging and Therapy. Aggregate, 2(6): e140.
Romanyuk AV, Grozdova ID, Ezhov AA, et al., 2017, Peroxyoxalate Chemiluminescent Reaction as a Tool for Elimination of Tumour Cells Under Oxidative Stress. Scientific Reports, 7(1): 3410.
Delafresnaye L, Bloesser FR, Kockler KB, et al., 2020, All Eyes on Visible-Light Peroxyoxalate Chemiluminescence Read-Out Systems. Chemistry–A European Journal, 26(1): 114–127.
Kuntzleman TS, Rohrer K, Schultz E, 2012, The Chemistry of Lightsticks: Demonstrations to Illustrate Chemical Processes. Journal of Chemical Education, 89(7): 910–916.
Skjolding LM, Dyhr KS, Köppl CJ, et al., 2021, Assessing the Aquatic Toxicity and Environmental Safety of Tracer Compounds Rhodamine B and Rhodamine WT. Water Research, 197: 117109.
Jameson DM, 2014, Introduction to Fluorescence, Taylor & Francis, Oxfordshire.
Kristoffersen AS, Erga SR, Hamre B, et al., 2014, Testing Fluorescence Lifetime Standards Using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow, Journal of Fluorescence, 24(4): 1015–1024
Huan B, Li J, Qu L, 2005, Study on the Mechanism of RhB by Fluorescence Spectroscopy. Journal of Tianjin Normal University (Natural Science Edition), 25(3): 8–10.
Wei Y, Kang Z, Liu C, et al., Resonance Light Scattering of Rhodamine B, Spectroscopy and Spectral Analysis 24(12): 1659–1662.
Ju M, Wang H, Xu P, et al., 2016, An Effect of Concentration on the Fluorescence Intensity of RhB Aqueous Solution. China’s Manganese Industry, 34(4): 79–82.
Chen RF, Knutson JR, 1988, Mechanism of Fluorescence Concentration Quenching of Carboxyfluorescein in Liposomes: Energy. Analytical Biochemistry, 172(1): 61–77.
Bakkialakshmi S, Selvarani P, Chenthamarai S, 2012, Fluorescence Quenching of Rhodamine B Base by Two Amines, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 105: 557–562.
Jenie SA, Kusumastuti Y, Krismastuti FS, et al., Rapid Fluorescence Quenching Detection of Escherichia Coli Using Natural Silica-Based Nanoparticles. Sensors, 21(3): 881.
Al-Hamedi FH, Rauf MA, Ashraf SS, 2009, Degradation Studies of Rhodamine B in the Presence of UV/H2O2. Desalination, 239(1–3): 159–166
Wang X, Wang J, Guo P, et al., 2009, Degradation of Rhodamine B in Aqueous Solution by Using Swirling Jet-Induced Cavitation Combined with H2O2. Journal of Hazardous Materials, 169(1–3): 486–491.
Kang K, Liu Q, Liu W, et al., 2015, Research on Degradation of Rhodamine B by Electro-fenton. Modern Agricultural Science and Technology, 2015(08): 223–224 + 226.
Liang L, Cheng L, Zhang Y, et al., 2015, Efficiency and Mechanisms of Rhodamine B Degradation in Fenton-like Systems Based on Zero-Valent Iron. RSC Advances, 10(48): 28509–28515.