Keywords
Fermented condition
Immune parameters
Intestinal flora
Submitted : 2025-07-15
Accepted : 2025-07-30
Published : 2025-08-14
Abstract
In this research, 8 strains with the capability to produce digestive enzymes or against pathogens were isolated from the shrimps’ gut. To obtain fermented feed with low pH, high content of acid-soluble protein, or high number of viable bacteria, the combination of strain, fermentation conditions was investigated. Then, a six-week feeding experiment in shrimps was conducted using 4 diets (F0: feed without fermentation; F1: fermented feed with low pH; F2: fermented feed with high content of viable bacteria; F3: fermented feed containing acid-soluble protein). At 21 days, T-AOC and SOD in F2 and F3 were significantly higher (P < 0.05) than those in the control group; GSH-Px activity in the F1 group and F3 group increased significantly; there are no significant differences (P > 0.05) among the four groups in GOT and GPT. The bacterial groups of the 4 experimental groups in the Phylum are composed mainly of Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes, and the F1 group had the highest relative proportion of Firmicutes.
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