Keywords
NSP1
Spike
RT-LAMP
Submitted : 2024-06-18
Accepted : 2024-07-03
Published : 2024-07-18
Abstract
Coronavirus disease (COVID-19) is a serious respiratory disease that spreads through the coronavirus globally. It soon became a pandemic after its appearance in 2019 and demanded new techniques for its identification and detection. Owing to this situation, RT-LAMP appears to be a novel method for the identification of COVID-19 because of its vast applications, including cost-effectiveness and time-saving. This research highlights the use of RT-LAMP, a more sensitive test than RT-PCR, for the assessment of SARS-CoV-2, the severe acute respiratory illness. To identify the spike (S) and NSP1 protein using RT-LAMP, 170 total samples of coronavirus-suspected patients were served in this research. Health certifications and bioethical considerations were taken into consideration. After the sample was extracted from the patient’s swabs, RNA was isolated, extracted, and purified. The response was then run on the RT-LAMP at the ideal temperature, and the outcomes could be observed with the unaided eye as they changed from pink to yellow. It is a simple method of determining if the test is positive or negative. For this purpose, both RT-LAMP and RT-PCR tests are used during these procedures. Genes linked with COVID-19 testing including S, nsp1, and ORF are suited to coronavirus testing; they have 100% specificity and low sensitivity, but S has more specificity and sensitivity than nsp1 and ORF, respectively. Out of the 95 positive samples, 89 (93.68%) samples yielded favorable outcomes utilizing RT-LAMP, while 55 negative samples yielded 100% positive results. The present research demonstrates that RT-LAMP is less sensitive yet more selective for coronavirus detection.
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