Tissue Regeneration in Infected Wounds of Albino Rats Using Ciprofloxacin-Loaded Gelatin Microspheres Incorporated Collagen Scaffold: A Histological Approach with H&E Staining

Abstract

A wound care system consisting of ciprofloxacin-loaded gelatin microspheres impregnated in a macroporous collagen scaffold was created to effectively control wound infection and regenerate soft tissue at the wound site. Histological and biochemical alterations were observed in infected wounds treated with these scaffolds in Albino Wistar rats. Furthermore, the study examined the immediate and prolonged release of ciprofloxacin from the scaffolds, as well as their function in eliminating bacterial infections and expediting the process of skin healing and regeneration. The developed technique was followed in the streamlined process of creating these collagen scaffolds. Compared to untreated wounds, the group receiving scaffold treatment experienced a faster rate of wound closure. It was noted that the rate of infections was considerably reduced and that full soft tissue regeneration occurred within 12 days. The development of well-deposited collagen bundles in the treated groups was demonstrated by H&E staining, which verified the flawless regeneration of the dermis and epidermis. The antimicrobial agent-loaded gelatin microspheres impregnated into the porous collagen scaffold demonstrated remarkable soft tissue regeneration and efficient infection control at the wound site.