Keywords
(R,S)-Goitrin
TLC
HPLC
Content determination
Submitted : 2024-02-18
Accepted : 2024-03-04
Published : 2024-03-19
Abstract
Objective: To establish the quality standards for the preparation of Xiao’er Qingre enema in hospitals. Method:Thin-layer chromatography (TLC) was used to identify Radix Isatidis and Glycyrrhizae in the prescription. The content of (R,S)-Goitrin was determined by high-performance liquid chromatography (HPLC). Results: In TLC identification, there was no interference between the negative controls of Radix Isatidis and glycyrrhizae, and the spots were well-separated. The HPLC results of (R,S)-Goitrin showed a good linear relationship between the peak area and concentration within the range of 0.476–95.2 μg·mL-1 (r = 0.9999). Conclusion: The TLC and HPLC methods established in this experiment are simple, reproducible, and specific, making them suitable for the quality control Xiao’er Qingre enema preparation in hospitals.
References
Zhang CH, 2019, Epidemiology and Clinical Characteristics of Hand, Foot and Mouth Disease. World Latest Medicine Information, 19(41): 228–229.
Ren J, Huang R, 2018, Epidemic Status and Prevention and Control Measures of Hand, Foot and Mouth Disease. China Health Industry, 15(26): 150–151.
Jiang RM, Deng HL, Li XM, et al., 2018, Interpretation of the Diagnosis and Treatment Guidelines for Hand, foot and Mouth Disease (2018 edition): General Treatment and Etiological Treatment of Hand, Foot and Mouth Disease. Infectious Disease Information, 31(5): 421–424.
Li F, Tian J, Wang J, et al., 2019, Research Progress of Rectal Drug Delivery System. Chinese Traditional Patent Medicine, 41(5): 1115–1118.
Jin FH, 2016, Determination of Water Extract (R-S)-Chuyichun from Radix Isatis by High-Performance Liquid Chromatography. China Medicine and Pharmacy, 12(6): 57–60.
National Pharmacopoeia Commission, 2015, Pharmacopoeia of the People’s Republic of China (Part I), China Medical Science Press, Beijing, 205–206.
Wu G, 2018, Analysis on the Content of Effective Components in Radix Isatis from Different Producing Areas. Forum on Traditional Chinese Medicine, 33(2): 63–64.
Li Z, Li L, Zhou H, et al., 2017, Radix Isatidis Polysaccharides Inhibit Influenza a Virus and Influenza a Virus-Induced Inflammation via Suppression of Host TLR3Signaling In Vitro. Molecules, 22(1): 116.
Gai Q-Y, Jiao J, Luo M, et al., 2015, Establishment of Hairy Root Cultures by Agrobacterium Rhizogenes Mediated Transformation of Isatis tinctoria L. for the Efficient Production of Flavonoids and Evaluation of Antioxidant Activities. PLoS One, 10(3): 1–15.
Chen R, Wang T, Li K, et al., 2019, Characteristics and Application of Immunomodulatory Antiviral Chinese Medicines. Chinese Traditional and Herbal Drugs, 51(06): 1412–1426.
Xu LH, Huang F, Chen T, et al., 2005, Antiviral Active Ingredients in Radix Isatis. Chinese Journal of Natural Medicines, 3(6): 359–360.
Su JH, Diao RG, Lv SG, et al., 2016, Modes of Antiviral Action of Chemical Portions and Constituents from Woad Root Extract against Influenza Virus A FM1. Evid Based Complement Alternat Med, 2016: 2537294.