Objective: To evaluate the pain degree of the patients with chronic non-cancer pain by using the evaluation model constituted by heart rate variability, anxiety and depression scale and quality of life rating scale, and to evaluate the efficacy after treatment and nursing intervention. Methods: 100 patients with chronic non-cancer pain treated in our hospital from February 2016 to April 2017 were selected to compare their heart rate variability, score of anxiety and depression, score of quality of life and NRS score before and after treatment and nursing intervention. Results: After treatment and nursing intervention, the heart rate variability time domain SDNN increased and the difference is significant (P < 0.05); the score of anxiety and depression was lower than that before intervention (P < 0.05); the scores of various dimensions of quality of life were higher than those before intervention (P < 0.05); the NRS score was lower than that before intervention (P < 0.05). Conclusion: The evaluation model constituted by heart rate variability, anxiety and depression scale and quality of life rating scale can be used to evaluate the pain degree of the patients with chronic non-cancer pain and to evaluate the efficacy after treatment and nursing intervention, which is worthy of clinic application.Â