Objective: To investigate the cognition, application and commendation of menopausal hormone therapy (MHT) among medical institution staff with different professional background. Methods: Female staff aged 40-60 years in 4 tertiary hospitals were randomly selected. The participants’ characteristics and the information of cognition, application and recommendation of MHT were collected by questionnaire. Results: A total of 341 people participated in this study, the cognition rate of MHT was 37% and the recommendation rate was 44%. For people with indications of MHT, the utilization rate is 15%, and 84.6% of them have used MHT for less than 3 years. 84.6% of those who did not use MHT considered menopause is not a disease and there was no need to use medication. Compared with nurses, doctors know more about MHT (P<0.01), and MHT recommendation rate of doctors was higher than that of nurses(P<0.01). The cognition of MHT were analyzed in menopause group, irregular menstruation group and regular menstruation group, there was no difference was found among these three groups (P>0.05). Conclusion: Strengthening the training of MHT related knowledge in medical institutions, will be helpful to improve the cognition and utilization rate of medical institutions and promote the whole society to study and use MHT.