Effects of Zhuang Medicine Longzuan Tongbi Decoction on Left Ventricular Remodeling in Rats with Myocardial Infarction

Abstract

Objective: To investigate the effects of Longzuan Tongbi Decoction on ischemic myocardial angiogenesis in rats with myocardial infarction (MI), and to analyze its regulatory effect on the expression of angiogenesis-related factors. Methods: A total of 120 healthy, clean-grade female Sprague-Dawley (SD) rats were randomly divided into six groups (20 rats per group): sham surgery (normal control) group, model group, Shexiang Baoxin Pill (positive control) group, as well as low-, medium-, and high-dose groups of Longzuan Tongbi Decoction. The MI model was established by left coronary artery ligation; the sham surgery group underwent threading without ligation. Postoperatively, each treatment group received daily oral administration of the corresponding medicine (the low-, medium-, and high-dose groups of Longzuan Tongbi Decoction received 1 mL, 2 mL, and 4 mL of the extract, respectively; the positive control group received Shexiang Baoxin Pill), while the sham surgery and model groups received an equal volume of saline. Treatment continued for 6 weeks. At the end of the experiment, the ratio of myocardial infarct size to left ventricular area was measured, and the number and density of microvessels in the MI border zone were assessed. Results: Compared with the model group, the ratio of myocardial infarct size to left ventricular area was significantly reduced in each dose group of Longzuan Tongbi Decoction (p < 0.05), and the efficacy was comparable to that of the Shexiang Baoxin Pill group. Regarding angiogenesis promotion, the number and density of microvessels in the infarct border zone were significantly higher in each dose group of Longzuan Tongbi Decoction than in the model group (p < 0.05); among these, the pro-angiogenic effect of the medium- and high-dose groups was significantly superior to that of the Shexiang Baoxin Pill group (p < 0.05), while no significant difference was observed between the low-dose group and the positive control group (p > 0.05). Conclusion: Longzuan Tongbi Decoction can effectively reduce the extent of myocardial infarction and exert a cardioprotective effect by promoting local angiogenesis in ischemic myocardium, and this effect exhibits a dose-dependent relationship within a certain range.