Objective: The aim of this study was to evaluate the effects and effectiveness of antenatal Kegel exercise on prevention of postpartum stress urinary incontinence. Methods: Totally 92 pregnant women who had Perinatal care card in Nanjing General Hospital of Nanjing Military Command were randomly divided into two groups. The pregnant women in the control group received routine health education, while the pregnant women who has 37 weeks of pregnancy in the experimental group received antenatal Kegel exercise and motivational interviewing intervention. The experimental group exercise 150-200 times every day. The incidence of SUI were measured after puerperae deliveried 42 days and 3 months. Results: The incidence of SUI was much more lower in the experimental group; the significant difference was found between the two groups. Conclusion: Antenatal Kegel exercise is more effective than routine health education on the prevention of postpartum stress urinary incontinence.