Objective. In order to explore the application effect of blood glucose management mode of multi-team cooperation in perioperative period of general surgery patients. Methods. The perioperative blood glucose control of 94 patients undergoing surgical treatment in general surgery from January 2016 to March 2019 was reviewed by Non-synchronous. According to the perioperative blood glucose management model of multi-team cooperation, the patients were divided into intervention group and control group. From January 2016 to January 2018, 64 patients who did not implemented the multi-team blood glucose management model were the control group and from February 2018 to March 2019, 30 patients who implemented the multi-team blood glucose management model were the intervention group, compared with two group about the differences in perioperative blood glucose. Results. It is no statistically significant about two group in highest and lowest blood sugar levels under fasting stats; during the fluid diet the blood sugar level of the intervention group was lower than control group(P<0.05),and when the patients was in the semi-fluid or food-feeding period, the highest and lowest blood sugar level is that the intervention group was lower than control group(P<0.05). The time of the blood sugar reaching the standard, the coincidence of complications rate and average hospitalization days in the intervention group were particularly lower than control group(P<0.05). Conclusion. If we adopt multi-team blood glucose management model can better control the perioperative blood glucose of patients undergoing general sugar.