Keywords
Gene
Multidrug-resistant pathogens
PCR
Submitted : 2024-06-18
Accepted : 2024-07-03
Published : 2024-07-18
Abstract
Antibiotics have been a cornerstone of treatment approaches as they protect against bacteria; however, they are often used without checks and balances. Over time, these antibiotics have become less effective due to bacterial resistance mechanisms. Through natural selection and random mutation, bacterial pathogens have developed resistance against several classes of antibiotics. One such resistance is beta-lactam resistance, caused by gene variants including the NDM gene. To date, comprehensive studies on this gene and its variants are limited in Pakistan. In our present study, multiple antibiotics were used to determine the susceptibility of bacterial samples, including Acinetobacter, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Escherichia coli. 82% of the samples were bla-NDM gene positive, as determined through molecular characterization using multiplex PCR. Further studies should be conducted on a larger sample scale to obtain more impactful results regarding antibiotic resistance in various bacteria.
References
Aminov RI, 2010, A Brief History of the Antibiotic Era: Lessons Learned and Challenges for the Future. Front Microbiol, 1: 134. https://doi.org/10.3389/fmicb.2010.00134
Ventola CL, 2015, The Antibiotic Resistance Crisis: Part 1: Causes and Threats. P T, 40(4): 277–283.
Davies J, Davies D, 2010, Origins and Evolution of Antibiotic Resistance. Microbiol Mol Biol Rev, 74(3): 417–433. https://doi.org/10.1128/MMBR.00016-10
Nikaido H, 2009, Multidrug Resistance in Bacteria. Annu Rev Biochem, 78: 119–146. https://doi.org/10.1146/annurev.biochem.78.082907.145923
Bush K, Fisher JF, 2011, Epidemiological Expansion, Structural Studies, and Clinical Challenges of New B-Lactamases from Gram-Negative Bacteria. Annu Rev Microbiol, 65: 455–478. https://doi.org/10.1146/annurev-micro-090110-102911
Nahid F, Khan AA, Rehman S, et al., 2013, Prevalence of Metallo-B-Lactamase NDM-1-Producing Multi-Drug Resistant Bacteria at Two Pakistani Hospitals and Implications for Public Health. J Infect Public Health, 6(6): 487–493. https://doi.org/10.1016/j.jiph.2013.06.006
Hetta HF, Ramadan YN, Al-Harbi AI, et al., 2023, Nanotechnology as a Promising Approach to Combat Multidrug Resistant Bacteria: A Comprehensive Review and Future Perspectives. Biomedicines, 11(2): 413. https://doi.org/10.3390/biomedicines11020413
Zhou G, Guo S, Luo Y, et al., 2014, NDM-1-Producing Strains, Family Enterobacteriaceae, in Hospital, Beijing, China. Emerg Infect Dis, 20(2): 340–342. https://doi.org/10.3201/eid2002.121263
Mohammadpour D, Memar MY, Kafil HS, et al., 2024, Detection of Carbapenemases Activity in MDR Isolates of Klebsiella pneumoniae by mCIM Method and Carbapenem Resistance Genes blaVIM, blaIMP, blaNDM, blaKPC-2 and blaOXA-48. Research Square, preprint. https://doi.org/10.21203/rs.3.rs-3998636/v1
Rani K, Gadekar HB, Tripathi A, 2023, Evaluation of Three Different Phenotypic Tests for Detection of Metallo-Beta-Lactamase in Imipenem Resistant Pseudomonas aeruginosa from Clinical Samples. Pakistan Heart Journal, 56(3): 204–212.
Ain NU, Iftikhar A, Bukhari SS, et al., 2018, High Frequency and Molecular Epidemiology of Metallo-B-Lactamase-Producing Gram-Negative Bacilli in a Tertiary Care Hospital in Lahore, Pakistan. Antimicrob Resist Infect Control, 7: 128. https://doi.org/10.1186/s13756-018-0417-y
Fallah F, Noori M, Hashemi A, et al., 2014, Prevalence of blaNDM, blaPER, blaVEB, blaIMP, and blaVIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran. Scientifica (Cairo), 2014: 245162. https://doi.org/10.1155/2014/245162
Hasan B, Perveen K, Olsen B, et al., 2014, Emergence of Carbapenem-Resistant Acinetobacter baumannii in Hospitals in Pakistan. J Med Microbiol, 63(Pt 1): 50–55. https://doi.org/10.1099/jmm.0.063925-0
Baran I, Aksu N, 2016, Phenotypic and Genotypic Characteristics of Carbapenem-Resistant Enterobacteriaceae in a Tertiary-Level Reference Hospital in Turkey. Ann Clin Microbiol Antimicrob, 15: 20. https://doi.org/10.1186/s12941-016-0136-2
Khan E, Irfan S, Sultan BA, et al., 2016, Dissemination and Spread of New Delhi Metallo-Beta-Lactamase-1 Superbugs in Hospital Settings. J Pak Med Assoc, 66(8): 999–1004.
Elbadawi HS, Elhag KM, Mahgoub E, et al., 2021, Detection and Characterization of Carbapenem Resistant Gram-Negative Bacilli Isolates Recovered from Hospitalized Patients at Soba University Hospital, Sudan. BMC Microbiol, 21(1): 136. https://doi.org/10.1186/s12866-021-02133-1