Comparative Study on the Effects of Several Hepatoprotective Drugs on Acute Liver Injury in Mice

Abstract

Objective: To investigate the protective effects of Gantaile capsule, ammonium glycyrrhizinate injection, and Ginseng Jianpi pill on CCl₄-induced acute liver injury in mice. Methods: Forty-eight mice were randomly divided into blank group, model group (15% CCl₄, 10 ml/kg), Gantaile group (150 mg/kg), ammonium glycyrrhizinate group (10 ml/kg), Ginseng Jianpi pill group (150 mg/kg), and positive control group (biphenyl diester, 150 mg/kg), with 8 mice in each group. Each administration group was given the corresponding dose of drugs by gavage, and the blank and model groups were given the corresponding volume of normal saline by gavage, once a day for 14 hours. Two hours after the last administration, the normal group was intraperitoneally injected with normal saline, and the other groups were intraperitoneally injected with CCl₄ peanut oil solution. 24 hours later, blood was collected from the eyeballs to measure the serum levels of alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor-alpha, and interleukin-6. The liver tissue was taken out to calculate the liver index. The morphological changes of the liver tissue and the degree of liver injury were observed by HE staining, and the activities of superoxide dismutase and malondialdehyde in the liver tissue were detected. Results: Several hepatoprotective drugs can significantly reduce CCl₄-induced acute liver injury in mice. Gantaile capsule has the most obvious effect, followed by ammonium glycyrrhizinate injection, and Ginseng Jianpi pill is the last. Conclusion: This experiment found that Gantaile capsules acted quickly, significantly alleviating liver injury. Ammonium glycyrrhizinate injection demonstrated the best anti-inflammatory effect. Compared to the first two drugs, Ginseng Jianpi pills showed less ideal effects, serving only as an auxiliary treatment.