Keywords
Bacteria
Glyceride
16S rDNA
Rhodococcus
Submitted : 2026-01-25
Accepted : 2026-02-09
Published : 2026-02-24
Abstract
Lipase, as an important biological catalyst, hydrolyzes triglycerides into fatty acids, diglycerides, monoglycerides, and glycerol, and is widely used in food, leather, pharmaceutical, and daily chemical industries, particularly in the food sector. Lipase is abundantly present in microorganisms and animals, with lipase-producing microorganisms being suitable for large-scale industrial production due to their rapid reproduction, short cycles, and diverse species. However, since there are currently few strains capable of producing high lipase activity, the increasing demand for industrial applications has made the search for more novel lipase-producing strains increasingly crucial. In this study, from 15 bacterial strains initially isolated from soil in the laboratory, one lipase-producing strain, zjy1-5, was identified through preliminary screening on glycerol-containing plates. Through 16S rDNA sequence analysis and Gram staining microscopic observation, the strain was identified as Rhodococcus rhodochrous, a Gram-positive bacterium. Subsequently, the fermentation conditions for lipase production were optimized, revealing that glucose was the optimal carbon source and 37°C the optimal culture temperature for lipase production. This research provides an experimental basis for further development and utilization of bacterial lipase.
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