Objective: This study focused on the clinical application value of dynamic CT perfusion imaging in stroke. Methods: A total of 92 patients with stroke were enrolled in this study. All patients were selected from December 2017 to December 2018 according to different diagnostic methods. They were randomly divided into two groups: the observation group and control group. The number of patients in each group was 46. The observation group mainly used dynamic CT perfusion imaging for diagnosis while the control group mainly used CT plain scan. The diagnostic effects of the two groups of patients and the hemodynamic parameters of the surrounding area of sub-acute hematoma and the acute phase of stroke in the observation group were compared. Results: The clinical diagnosis of the observation group was 95.65% while the clinical diagnosis of the control group was 69.57%. The observation group was higher than that of the control group and the difference was significant. In addition, the hemodynamic parameters of the acute phase of the observation group and the sub-acute hematoma were also varied. Conclusion: Dynamic CT perfusion imaging has significant clinical value in stroke, and it is worthy of further application.