Significance of EZH2 and BMI-1 Protein Expression in Carcinogenesis of Colon Serrated Adenoma
PDF

Keywords

Serrated adenoma of colon
EZH2
BMI-1

DOI

10.26689/jcnr.v5i4.2271

Abstract

Objective: To observe the significance of EZH2 and BMI-1 protein expression in the carcinogenesis process of colon serrated adenoma (SSA/P). Methods: Hematoxylin-eosin (HE) staining was used to observe the morphological characteristics of normal tissues, hyperplastic polyp (HP), SSA/P and colon cancer. Immunohistochemical staining was used to detect the expression of EZH2 and BMI-1 protein. The relative expression of EZH2 and BMI-1 was detected by qRT-PCR. Results: Compared with normal tissues, HP and colon cancer tissues, SSA/P showed serrated glandular hyperplasia, glandular dilatation, and deep nuclear staining, which had certain atypia. The positive expression rates of EZH2 and BMI-1 protein were 53.3% and 56%, which were close to those of colon cancer (66.7% and 76.6%) and higher than those of normal group and HP (16% and 8%, P < 0.05). The relative expression of EZH2 and BMI-1 in SSA/P tissue was significantly higher than that in normal group and HP, but lower than that in carcinogenesis group (P<0.05). Conclusion: EZH2 and BMI-1 play an important role in the carcinogenesis of colon serrated adenoma, and can be used as the primary screening index before carcinogenesis.

References

Steele JC, Torr EE, Noakes KL, et al., 2006, The Polycomb Group Proteins, BMI-1 and EZH2, are Tumour-Associated Antigens. British Journal of Cancer.

Bao H, Li J, Xing G, 2014, Expression and Significance of EZH2 and Bmi-1 Genes in Colorectal Cancer Tissues. Heilongjiang Medical Science, 37(1):10-12.

Brody H., 2015, Colorectal Cancer. Nature. 521(7551): S1.

Gan L, Yang Y, Li Q, et al., 2018, Epigenetic Regulation of Cancer Progression by EZH2: from Biological Insights to Therapeutic Potential. Biomarker Research, 6(1):10.

Pourjafar M, Samadi P, Karami M, et al., 2020, Assessment of Clinicopathological and Prognostic Relevance of BMI 1 in Patients with Colorectal Cancer: A Meta Analysis. Biotechnology and Applied Biochemistry.

Suva ML, Riggi N, Janiszewska M, et al., 2009, EZH2 is Essential for Glioblastoma Cancer Stem Cell Maintenance. Cancer Research, 69(24):9211-9218.

Yu J, Chen L, Bao Z, et al., 2020, BMI1 Promotes Invasion and Metastasis in Endometrial Adenocarcinoma and is a Poor Prognostic Factor. Oncology Reports.

Pan H, Hou J, He J, et al., 2010, Expression and Significance of EZH2 and Bmi-1 Genes in Transitional Cell Carcinoma of the Bladder. Chinese Journal of Experimental Surgery, 27(3):292-294.

Shao G, Zhang G, Pu H, et al., 2015, Expression and Clinical Significance of Bmi-1, NANOG, EZH2 in Colorectal Cancer. Journal of Xinjiang Medical University, 38(3): 350-350.