Objective: To explore the implementation effect of nursing management in operating room for hospital infection control. Methods: 100 cases of patients who received surgical treatment in our hospital from 2019 to 2020 were selected and divided into observation group and control group according to different nursing management methods in operating room. Patients in the control group received routine nursing management services, while patients in the observation group received systematic and high-quality comprehensive management services based on routine nursing management services. Results: The air colony count and hand colony count of the observation group were significantly better than those of the control group. At the same time, the incidence probability of infection in the observation group was also significantly lower than that of the control group, with significant difference between the groups, with statistical value (P < 0.05). Conclusions: In view of the occurrence of nosocomial infection, the implementation of high-quality operating room nursing service can effectively control it, reduce the probability of occurrence, and ensure the safety of patient treatment, which is worthy of clinical application.