Study on the Changes of Serum Vaspin Level and its Regulatory Mechanism in Patients with Thyroid Dysfunction

Abstract

Objective: To study the changes of serum vaspin levels in hyperthyroidism and hypothyroidism, and the correlation between serum vaspin and FT3, FT4, TSH and HOMA-IR. Methods: According to the diagnostic criteria of hyperthyroidism and hypothyroidism published in the 8th edition of internal medicine, the patients were divided into hyperthyroidism group (n = 47), male 14, female 33, average age (35 ± 9) years; hypothyroidism group: 23 hypothyroidism patients, 7 males and 16 females, with an average age of (38 ± 10) years. The blood pressure, height and weight of all the participants were measured by a specially assigned person, and the body mass index (BMI = weight (kg) / height (M2) and ankle brachial index (ABI) were calculated. Venous blood samples were drawn from all subjects after fasting for 8 hours in the morning to determine biochemical indexes. Fasting insulin (fins) was measured by chemiluminescence method, insulin resistance index (HOMA-IR, HOMA-IR = FPG × fins / 22.5) was calculated by homeostasis model assessment (HOMA-IR), and HbA1c was determined by high-pressure liquid chromatography. The levels of FT3, FT4 and TSH were detected by radioimmunoassay. Serum vaspin levels were measured by ELISA. Results: The level of BMI in hypothyroidism group was significantly higher than that in hyperthyroidism group and control group (P < 0.01), BMI level in hyperthyroidism group was significantly lower than that in control group (P < 0.05), FT3 and FT4 levels in hyperthyroidism group were significantly higher than those in hypothyroidism group and control group (P < 0.01), TSH level in hypothyroidism group was significantly higher than that in control group and hyperthyroidism group (P < 0.01). The level of FPG in hyperthyroidism group was significantly higher than that in control group (P < 0.01), but there was no significant difference between hyperthyroidism group and hypothyroidism group, and fins level in hypothyroidism group was significantly higher than that in control group and hyperthyroidism group (P < 0.01). The level of HOMA-IR in hyperthyroidism and hypothyroidism group was significantly higher than that in control group (P < 0.01). Compared with the control group and the control group, the blood lipid indexes (TC, LDL-C) in the hyperthyroidism group were lower than those in the control group and hypothyroidism group (P<0.01), and all the blood lipid indexes in the hypothyroidism group were significantly different from those in the control group (P<0.01). The vaspin level of hyperthyroidism group was significantly higher than that of control group and hypothyroidism group, and the latter two groups showed that the level of vaspin in hypothyroidism group was significantly lower than that of control group (P < 0.05). Correlation analysis showed that serum vaspin was positively correlated with FT3 and FT4 (r = 0.255, P = 0.005; r = 0.327, P = 0.001), and negatively correlated with BMI, TC and HDL (r = -0.250, P = 0.006; r = -0.244, P = 0.007; r = -0.258, P = 0.004). )   Conclusion: Serum vaspin level is related to thyroid function. The level of serum vaspin increases in hyperthyroidism and decreases in hypothyroidism. Abnormal changes of fat factor vaspin are associated with thyroid dysfunction.