Objective: To investigate timing and clinical efficacy of laparoscopic cholecystectomy for acute cholecystitis at different stages. Methods: Clinical data of 100 acute cholecystitis patients admitted to our hospital from March 2018 to March 2019 were retrospectively analyzed. Clinical data of 48 patients who had surgery within 72 hours of symptom onset were classified as group A, and clinical data of 52 patients who had surgery at ? 72 hours of symptom onset were classified as group B. Clinical indicators, rate of conversion to open surgery, and complication were compared between the two groups. Results: Length of hospital stay, operation time, exhaust time and intraoperative blood loss in group A were lower than those in group B, and the differences were statistically significant (P<0.05). Comparison of rate of conversion to open surgery between group A (2.08%, 1/48) and group B (7.69%, 4/52) showed no statistically significant difference (P>0.05). Incidence of postoperative complication was 8.33% (4/48) in group A and 11.54% (6/52) in group B, the difference was not statistically significance (P>0.05). Conclusions: Laparoscopic cholecystectomy within 72 hours of symptom onset has better efficacy than after 72 hours for patients with acute cholecystitis. It could effectively reduce surgical trauma and promote recovery.