摘要
Objective: To investigate the application of liquid chromatography-mass spectrometry (LC-MS) in the determination of chemical constituents of kidney tonic and aphrodisiac Chinese herbal preparations. Methods: In this study, 30 kidney tonic and aphrodisiac Chinese herbal preparations were selected as experimental materials, and chromatography and LC-MS were used to detect their chemical compositions in the control group and the experimental group, respectively. The excellent rate of detection method, identification time, and accuracy of the two methods were compared. Results: The excellent rate of the detection method in the experimental group (93.33%) was significantly higher than that in the control group (73.33%), and the difference was statistically significant (P < 0.05); compared with the control group, the detection time and retention time error in the experimental group were shorter, and the difference showed highly significant correlation (P < 0.001). Conclusion: LC-MS is of high value in the detection of chemical constituents of kidney tonic and aphrodisiac Chinese herbal preparations, which can significantly improve the excellent rate of the detection method and the success rate, shorten the detection time, and reduce the retention time error, and is worth to be popularized.
参考
Xie R, Zhu Lg, Yu J, et al., 2020, A Study on the Mechanism of Action of Kidney Tonifying and Blood Activating Traditional Chinese Medicine in the Treatment of Degenerative Lumbar Spine Instability Based on Network Pharmacology. World Journal of Integrative Medicine, 15(11): 1992–1997 + 2006.
Meng X, Zhang W, Zhao C, et al., 2020, A Preliminary Study on the Network Molecular Mechanism of Kidney-Yang Tonifying Traditional Chinese Medicine in Treating Gonadotropin-Deficient Male Infertility. Global Chinese Medicine, 13(01): 29–34.
Wu C, Wei J, Chen H, et al., 2020, Prediction and Validation of the Biological Material Basis and Mechanism of Action of Kidney Tonifying and Essence Benefiting Traditional Chinese Medicines for Treating Diseases Related to Kidney Essence Deficiency Syndrome. Journal of Pharmacy, 55(03): 463–472.
Wu C, Zhang J, Liu Y, et al., 2017, Study on the Intervention Effect of Kidney Tonifying and Liver Cleansing Chinese Herbal Medicine Pairing on p38MAPK/NF-?B Signaling Pathway in an Intermittent Hypoxia-Induced Human Umbilical Vein Endothelial Cell Injury Model. Chinese Journal of Traditional Chinese Medicine, 42(23): 4661–4664.
Lu QY, He XS, Fang J, et al., 2022, Simultaneous Determination of Linezolid and Voriconazole Serum Concentrations Using Liquid Chromatography-Tandem Mass Spectrometry. J Pharm Biomed Anal, 2022(212): 114659.
Li Y, Song Y, Wang WT, et al., 2023, Application of Liquid Chromatography-Mass Spectrometry in Vaccine Analysis. China Food and Drug Regulation, 2023(07): 30–43 + 143–144.
Ji B, Yang L, Han Y, et al., 2023, Progress of Matrix Cleanup and Liquid Chromatography-Mass Spectrometry for Multi-Residue Detection of Veterinary Drugs in Foods of Animal Origin. Journal of Light Industry, 38(05): 8–16.
Fan X, Zhai R, Chu Z, et al., 2022, Progress in Quantification of Protein-Based Tumor Markers Based on Liquid Chromatography-Mass Spectrometry. Journal of Mass Spectrometry, 43(03): 263–277.