Keywords
Kidney stones
Oxidative stress
Lidan Huashi Pills
Rats
Submitted : 2024-06-24
Accepted : 2024-07-09
Published : 2024-07-24
Abstract
Objective: To explore the effects of Lidan Huashi Pills on the glutathione peroxidase (GSH-Px) activity and growth of calcium oxalate crystals in the kidneys of rats. Methods: 30 rats were randomly divided into a blank group, a model group, and an experimental group, with 10 rats in each group. The blank group was free to eat and drink water for 8 weeks; the ethylene glycol method was used for the standard calcium oxalate crystal modeling in the control group and experimental group for 4 weeks. The model group was given free feeding and physiological saline (2 ml/d) by continuous gavage for 4 weeks; the experimental group was given free feeding and Lidan Huashi Pills (450 mg/kg, 2 ml/d) by continuous gavage for 4 weeks. After 4 weeks, all rats were euthanized, and the left kidney was taken for GSH-Px level detection. The right kidney was stained with hematoxylin and eosin (H&E) to observe the formation of calcium oxalate crystals. Results: After 4 weeks of modeling, the urinary calcium levels in the model group and experimental group significantly increased compared to the blank group (868.00 ± 39.29 vs 929.40 ± 33.61, P < 0.05), indicating successful modeling. The urine calcium ion concentration in the experimental group after modeling was significantly lower than that in the model group (929.40 ± 33.61 vs 888.60 ± 25.92, P < 0.05). The grading score of calcium oxalate crystals in the model group was significantly higher than that in the blank group (P < 0.05); the grading score of calcium oxalate crystals in the experimental group was lower than that in the model group, and the difference was not statistically significant (P > 0.05). The GSH-Px activity in the left kidney of the model group was significantly lower than that of the blank group [203.49 (208.21, 144.22) vs 494.91 (431.32, 538.18), P < 0.05); the GSH-Px activity in the left kidney of the experimental group was significantly higher than that of the model group [433.60 (383.86, 504.49) vs 203.49 (208.21, 144.22), P < 0.05). Morphological observation and H&E staining suggest that the formation of right kidney crystals and inflammation in the experimental group are between the blank group and the model group. Conclusion: Lidan Huashi Pills can enhance the serum GSH-Px activity in rats and inhibit the growth of calcium oxalate crystals and inflammatory response in the kidneys, thus playing a role in preventing and treating urinary tract stones.
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