Keywords
Erythrocyte membrane permeability
Different species
Differential analysis
Submitted : 2025-12-13
Accepted : 2025-12-28
Published : 2026-01-12
Abstract
Through comparative studies on renal structure and urine concentration function, this research aims to observe and analyze the differential mechanisms among different animal species. Using rats as the research subjects and employing a high-protein feeding method, the study explores the mechanism of urea’s effect on urine concentration function from multiple levels, including gene expression regulation and protein levels. Erythrocyte membrane permeability tests are conducted to compare the degree of differences among various animal species. This study utilized experimental techniques such as RT-PCR, Western-blot, Stopped-flow, and immunohistochemistry to investigate the urine concentration mechanism at the cellular, tissue, and whole-organism levels, laying a foundation for further screening and preparing animal models for studying the urine concentration mechanism.
References
Jiang T, Yang B, 2015, Tissue Distribution and Physiological Functions of Urea Transporter Proteins. Acta Neuropharmacologica, 5(5): 40–48.
Li Y, Yang B, 2018, Research Progress on Renal Physiology of Urea Transporter Proteins. Acta Physiologica Sinica, 70(6): 649–656.
Wang S, 2001, Examination of Urine Concentrating Function and Its Influencing Factors. Chinese Journal of Nephrology, Dialysis and Transplantation, 2001(6): 564–568.
Liu L, 2013, Study on Differences in Urine Concentrating Function and Erythrocyte Membrane Permeability Among Different Animal Species, thesis, Jilin University.
Peterson M, Rishniw M, 2023, Urine Concentrating Ability in Cats with Hyperthyroidism: Influence of Radioiodine Treatment, Masked Azotemia, and Iatrogenic Hypothyroidism. Journal of Veterinary Internal Medicine, 37(6): 2039–2051.
Zhao W, Zhang J, 2016, Experimental Results and Analysis of Mouse Erythrocyte Membrane Permeability. Journal of Higher Education, (11): 106–107.
Liu M, Lu J, Chen Y, et al., 2023, Sodium Sulfite-Triggered Hepatocyte Ferroptosis via mtROS/Lysosomal Membrane Permeabilization-Mediated Lysosome Iron Efflux. Journal of Agricultural and Food Chemistry, 71(43): 16310–16322.
Swarnava R, Parul R, Marthe-Sandrine M, et al., 2018, Angiotensin Receptor Signaling in Sickle Cell Anemia Exerts a Renoprotective Effect on Urine Concentrating Ability but Leads to Sickle Glomerulopathy. American Journal of Hematology, 93(7): E177–E181.
De Groot T, Doornebal J, Christensen B, et al., 2017, Lithium-Induced Nephrogenic Diabetes Insipidus: Acetazolamide Reduces Polyuria but Does Not Improve Urine Concentrating Ability. American Journal of Physiology: Renal Physiology, 313(3): F669–F676.