Keywords
Human genetics
Chromosome territory
3D-FISH
Submitted : 2025-03-03
Accepted : 2025-03-18
Published : 2025-04-02
Abstract
The stability and evolution of human genetics depend on chromosomes and chromosome-chromosome interactions. We wish to understand the spatial location of chromosomes in dividing cells in order to understand the relationship between chromosome-chromosome interactions and to further investigate the role of chromosomes and their impact on cell biological behavior. In this study, we explored the relative spatial positional relationships of chromosomes [t(9;22) and t(15;17)] in B-ALL cells by using the three-dimensional DNA fluorescent in situ hybridization (3D-FISH) method. The results showed that chromosomes [t(9;22) and t(15;17)] showed relatively stable spatial relationships. The relative stability of the spatial location of chromosomes in dividing cells may be relevant to disease.
References
Cremer T, Cremer C, 2001, Chromosome Territories, Nuclear Architecture and Gene Regulation in Mammalian Cells. Nat Rev Genet, (2): 292–301. https://doi.org/10.1038/35066075
Lieberman-Aiden E, van Berkum NL, Williams L, et al., 2009, Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome. Science, (326): 289–293. https://doi.org/10.1126/science.1181369
Schmitt AD, Hu M, Ren B, 2016, Genome-Wide Mapping and Analysis of Chromosome Architecture. Nat Rev Mol Cell Biol, (17): 743–755. https://doi.org/10.1038/nrm.2016.104
Cremer T, Cremer M, Dietzel S, et al., 2006, Chromosome Territories: A Functional Nuclear Landscape. Curr Opin Cell Biol, (18): 307–316. https://doi.org/10.1016/j.ceb.2006.04.007
Kosak ST, Scalzo D, Alworth SV, et al., 2007, Coordinate gene Regulation During Hematopoiesis is Related to Genomic Organization. PLoS Biol, (5): e309. https://doi.org/10.1371/journal.pbio.0050309
Gerlich D, Beaudouin J, Kalbfuss B, et al., 2003, Global Chromosome Positions are Transmitted Through Mitosis in Mammalian Cells. Cell, (112): 751–764. https://doi.org/10.1016/s0092-8674(03)00189-2
Parada L, Misteli T, 2002, Chromosome Positioning in the Interphase Nucleus. Trends Cell Biol, (12): 425–432. https://doi.org/10.1016/s0962-8924(02)02351-6
Chess A, 2016, Monoallelic Gene Expression in Mammals. Annu Rev Genet, (50): 317–327. https://doi.org/10.1146/annurev-genet-120215-035120
Kosak ST, Skok JA, Medina KL, et al., 2002, Subnuclear Compartmentalization of Immunoglobulin Loci During Lymphocyte Development. Science, (296): 158–162 (2002). https://doi.org/10.1126/science.1068768
Soler G, Ouedraogo ZG, Goumy C, et al., 2023, Optical Genome Mapping in Routine Cytogenetic Diagnosis of Acute Leukemia. Cancers (Basel), 15(7): 2123. https://doi.org/10.3390/cancers15072131
Bontadini A, Iannelli S, Fruet F, et al., 2015, Erroneous HLA Typing as a Result of Acquired Uniparental Disomy in a Patient with Acute Lymphoblastic Leukaemia in Peripheral Blood Complete Remission. Blood Transfus, (13): 678–681. https://doi.org/10.2450/2015.0236-14
Pasquini L, Ponziani I, Spataro E, et al. Elevated Nuchal Translucency, is it Time to Discuss the Cut Off? Int J Gynaecol Obstet, 163(2): 540–546. https://doi.org/10.1002/ijgo.14834
de Kretser DM, 1997, Male Infertility. Lancet, (349): 787–790. https://doi.org/10.1016/s0140-6736(96)08341-9
Boyle S, Gilchrist S, Bridger JM, et al., 2001, The Spatial Organization of Human Chromosomes Within the Nuclei of Normal and Emerin-Mutant Cells. Hum Mol Genet, (10): 211–219. https://doi.org/10.1093/hmg/10.3.211
Jiang T, Wu C, Wajid A, et al., 2019, Arrangement of Human Cell Metaphase Chromosomes on the Equatorial Plate. Journal of Cell Science & Therapy, (10): 2. https://doi.org/10.35248/2157-7013.19.10.289
Ventura RA, JI Martin-Subero, Jones M, et al., 2006, FISH Analysis for the Detection of Lymphoma-Associated Chromosomal Abnormalities in Routine Paraffin-Embedded Tissue. J Mol Diagn, (8): 141–151. https://doi.org/10.2353/jmoldx.2006.050083
Wolff DJ, Bagg A, Cooley LD, et al., 2007, Guidance for Fluorescence In Situ Hybridization Testing in Hematologic Disorders. J Mol Diagn, (9): 134–143. https://doi.org/10.2353/jmoldx.2007.060128
Cremer M, von Hase J, Volm T, et al., 2001, Non-Random Radial Higher-Order Chromatin Arrangements in Nuclei of Diploid Human Cells. Chromosome Res, (9): 541–567. https://doi.org/10.1023/a:1012495201697
Parada LA, McQueen PG, Misteli T, 2004, Tissue-Specific Spatial Organization of Genomes. Genome Biol, (5): R44. https://doi.org/10.1186/gb-2004-5-7-r44