Effects of IL-6/JAK2/STAT3 on the Biological Behavior of Oral Squamous Cell Carcinoma
Download PDF


Oral squamous cell carcinoma



Submitted : 2023-06-20
Accepted : 2023-07-05
Published : 2023-07-20


Objective: To investigate the effect of interleukin 6/Janus kinase 2/signal transducer and activator of transcription 3 (IL-6/JAK2/STAT3) on the biological behavior of oral squamous cell carcinoma (OSCC). Methods: OSCC cells were transfected with the designed lentiviral vector plasmid pGMLV-SB3 (experimental group) and the corresponding negative control plasmid pGMLV-SB3-shNC (control group); 48 hours after transfection, a liposome transfection kit (Sigma, USA) was used for lentivirus packaging; after virus packaging, a medium containing pGMLV-SB3 lentiviral vector was added and cultured for 24 h; the cells were harvested, and RNA was extracted; Transwell chamber assay (Sigma, USA) was used to detect cell migration and invasion ability; dot-enzyme-linked immunosorbent assay (ELISA) kit was used to detect the level of interleukin 6 (IL-6) in the culture supernatant, while serum IL-6 level was measured by ELISA. Results: The expressions of IL-6, JAK2, and STAT3 in the experimental group were significantly raised, as compared to the control group (P < 0.05); the apoptosis rate of OSCC cells in the experimental group, which was detected by flow cytometry 48 h after transfection, was significantly higher than that of cells in the control group (P < 0.05); and there was a significant improvement in the experimental group’s cell migration and invasion ability, as compared to that of the control group (P < 0.05). Conclusion: The IL-6/JAK2/STAT3 signaling pathway plays an important role in the migration and invasion of OSCC cells. Inhibiting the expression of IL-6 can inhibit the growth and proliferation of OSCC cells as well as reduce their ability to invade and migrate. These results provide a new target for the treatment of OSCC.


Wang N, Jin W, Peng X, 2023, Research Progress of Human Xenograft Model of Oral Squamous Cell Carcinoma. Modern Stomatology, 37(3): 195–199.

Yang Y, Song X, Wu Y, 2023, Notch1 (P1641S) Mutation Promotes the Proliferation of Oral Squamous Cell Carcinoma Through PI3K/Akt Pathway. Stomatological Medicine, 43(5): 393–399.

Zhang H, Jiang Y, Sun L, et al., 2023, The Expression of SMARCA5 in Oral Squamous Cell Carcinoma and Its Effect on the Proliferation, Migration and Invasion of HSC4 Cells. Chongqing Medicine, 52(9): 1292–1297.

Wu R, Liu H, Zheng S, et al., 2023, MCU Maintains Mitochondrial Calcium Homeostasis Through LETM1 to Regulate Oral Squamous Cell Carcinoma Metastasis. Journal of Chongqing Medical University, 48(4): 411–416.

Zheng S, Meng L, Ren F, et al., 2023, Cell-Free DNA from Oral Squamous Cell Carcinoma Regulates Stemness and Migration of Oral Cancer Cell Lines by Inducing Macrophage Polarization. Journal of Sichuan University (Medical Edition), 54(3): 510–516.

Zhang C, Li C, 2023, Relationship Between TP53 Gene Mutation and Prognosis of Oral Squamous Cell Carcinoma. Modern Oncology, 31(11): 2169–2172.

Yang K, Sun Y, Hu Y, et al., 2023, The Predictive Effect of Cyclin D1 Expression Pattern Deep Learning Recognition and Prediction Model on the Prognosis of Patients with HPV-Negative Oral Squamous Cell Carcinoma and Oropharyngeal Squamous Cell Carcinoma. Stomatology Research, 39(4): 308–315.

Xu L, Huang Z, Lu D, et al., 2022, The MiR-663b/ALDH6A1 Axis Regulates the Proliferation of Oral Squamous Cell Carcinoma Cells Through ROS. Journal of Local Surgery, 31(9): 747–753.

Li W, Cao J, Guo X, et al., 2022, Tumor-Associated Macrophage-Derived IL-6 Promotes the Invasion and Migration of Oral Squamous Cell Carcinoma CAL27 Cells by Up-Regulating the Expression of LIF in Tumor Cells. Journal of Jilin University (Medical Edition), 48(4): 946–953.

Tong X, Zhou X, Zhong H, et al., 2022, Effects of IL-6/JAK2/STAT3 on Biological Behavior of Oral Squamous Cell Carcinoma. Chinese Journal of Otorhinolaryngology and Skull Base Surgery, 28(1): 89–97.

Qu Y, He Y, Yang Y, et al., 2019, Proceedings of the 2019 First National Oral and Maxillofacial - Head and Neck Tumor Academic Conference, December 20–22, 2019: ALDH3A1 as a Prognostic Marker for Oral Squamous Cell Carcinoma Inhibits the Epithelial-Mesenchymal Transition of Tumor Cells Through the IL-6/STAT3 Signaling Pathway. Convergence Leading, 94–95.

Zhang S, 2019, Bit-1 Regulates the Migration, Invasion and Apoptosis of Oral Squamous Cell Carcinoma Through the IL-6/IL-6R-STAT3 Pathway, thesis, Zhengzhou University.

Yang J, 2018, The Role of SOCS3 Gene Silencing in Regulating the Differentiation and Maturation of Dendritic Cells in Candida albicans Infection and Its Immunological Mechanism, thesis, Peking Union Medical College, Chinese Academy of Medical Sciences, Tsinghua University School of Medicine.

Xu X, Zhang R, 2014, Research Progress on the Correlation Between Interleukin-6 and Oral Diseases. Stomatological Medicine, 34(6): 470–472.

Du J, Yan Y, 2010, Detection of CEA, IL-6 and IL-8 in Saliva of Patients with Oral Squamous Cell Carcinoma. Journal of Anhui Medical University, 45(4): 579–581.