Keywords
Glomerulonephritis
NLRP3
Submitted : 2023-02-05
Accepted : 2023-02-20
Published : 2023-03-07
Abstract
Objective: To investigate the effect of the ethanolic extract of Rosa laevigata Michx. fruit on rats with mesangial proliferative glomerulonephritis based on the NLRP3 inflammasome pathway. Methods: Thirty Wistar rats were divided into three groups, a blank control group, a diabetic nephropathy (DN) model group, and an ethanolic extract intervention group, according to the random number table method, with 10 rats in each group. One day before the experiment, basic feeding was initiated for all the rats; the changes in activity and weight of each group of rats were observed and recorded after 7 d, and a rat model of renal function injury was established after 1 d. Results: Compared with the control group, the model group had significantly higher kidney/body ratio, 24 h urine protein, serum creatinine (SCr), blood urea nitrogen (BUN), glomerular mesangial cell (GMC) count, and extracellular matrix (ECM) positive area ratio (P < 0.05); the same indicators were significantly lower in the intervention group than in the model group (P < 0.05). The NLRP3 inflammasome pathway in renal intrinsic cells was activated in the intervention group. The overactivation of NLRP3 inflammasome is known to promote interleukin (IL)-1? release, which was inhibited in the intervention group. Conclusion: The ethanolic extract of Rosa laevigata Michx. fruit has a protective effect on renal intrinsic cells and may be related to NLRP3 inflammasome pathway, suggesting that the fruit of Rosa laevigata Michx. has a potential role in protecting renal intrinsic cells from inflammatory damage. NLRP3 inflammasomes are involved in the development of various chronic inflammatory diseases, such as acute and chronic glomerulonephritis and renal fibrosis.
References
Wang X, Wang A, Zhang Q, et al., 2017, Observation of Cyclosporine A Combined with Medium/Low Dose Prednisone in the Treatment of Progressive IgA Nephropathy. Journal of Pharmacoepidemiology, 26(3): 168–170.
Yamada Y, Endou M, Morikawa S, et al., 2018, Lactic Acid Bacteria Isolated from Japanese Fermented Fish (Funa-Sushi) Inhibit Mesangial Proliferative Glomerulonephritis by Alcohol Intake with Stress. J Nutr Metab, 2018: 6491907.
Yashima A, Mizuno M, Yuzawa Y, et al., 2016, Mesangial Proliferative Glomerulonephritis in Murine Malaria Parasite, Plasmodium Chabaudi AS, Infected NC Mice. Clin Exp Nephrol, 21(4): 589–596.
Huang K, Li R, Wei W, 2018, Sirt1 Activation Prevents Anti-Thy 1.1 Mesangial Proliferative Glomerulonephritis in the Rat Through the Nrf2 /ARE Pathway. Eur J Pharmacol, 832: 138–144.
Wang Y, Zhang L, Wang D, et al., 2017, Shendi Granules Regulates the Balance of T Cell Subsets and Down-Regulates Podocalyxin in Rats with Mesangial Proliferative Glomerulonephritis. Chin J Cell Mol Immunol, 33(8): 1014–1019.
He Y, Hara H, Núñez G, 2016, Mechanism and Regulation of NLRP3 Inflammasome Activation. Trends Biochem Sci, 41(12): 1012–1021.
Jo EK, Kim JK, Shin DM, et al., 2016, Molecular Mechanisms Regulating NLRP3 Inflammasome Activation. Cell Mol Immunol, 13(2): 148–159.
Chanrat E, Worawichawong S, Radinahamed P, 2018, Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Predictors of Complete Remission in Primary Glomerulonephritis. Cytokine, 104(4): 1–7.
Wang Y, Xu W, Wang D, et al., 2017, Interventional Effect of Gandhi Granules on Endothelial Cells in Rats with Thylakoid Proliferative Glomerulonephritis. Chinese Patent Medicine, 19(11): 2369–2372.
Chen L, Wang C, Luo Y, et al., 2015, Study on the Protective Effect of Water Extract of Plantain on Rats with Glomerulonephritis. Shizhen National Medicine, 26(12): 2874–2877.
Chen LY, Wang CQ, Luo YH, et al., 2015, Protective Effect of Plantain Extract on Glomerulonephritis in Rats. Lishizhen Med Materia Med Res, 26(12): 2874–2877.
Liu X, Hou X, Cui Q, et al., 2017, Optimization of Extraction of Polysaccharides from C. Chinensis and Its In Vitro Antiviral Activity. Chinese Medicinal Materials, 40(7): 1679–1682.
Liu XW, Hou L, Cui QH, et al., 2017, Study on the Extraction Optimization of RLMP and Its Antiviral Activity In Vitro. J Chin Med Materials, 40(7): 1679–1682.
Tao X, Sun X, Xu L, et al., 2016, Total Flavonoids from Rosa Laevigata Michx Fruit Ameliorates Hepatic Ischemia/Reperfusion Injury Through Inhibition of Oxidative Stress and Inflammation in Rats. Nutrients, 8(7): 268–274.
Wang YP, Xu WJ, Wang D, et al., 2017, Effect of Shendi Granule on Endothelial Cells with Mesangial Proliferative Glomerulonephritis. Chin Tradit Patent Med, 19(11): 2369–2372.