Keywords
A549
EMT
Lung cancer
Submitted : 2022-09-18
Accepted : 2022-10-03
Published : 2022-10-18
Abstract
Objective: To investigate the role of KIF3C gene in promoting the malignant phenotype of lung cancer cells and in regulating PI3K/AKT signaling pathway. Methods: CCK-8 and transwell assays were used to detect the changes in cell proliferation and cell migration ability after being transfected with siKIF3C, as well as the protein expression levels of PI3K, p-PI3K, AKT, and p-AKT following the downregulation of KIF3C by Western blot. Results: The CCK-8 assay showed that the proliferation/viability of lung cancer cells A549 significantly reduced after being transfected with siKIF3C gene (P < 0.05); the migration ability of lung cancer cells A549 was significantly reduced after transfected with siKIF3C gene (P < 0.05); the levels of p-PI3K and p-AKT proteins were downregulated after KIF3C protein knockdown (P < 0.05); however, the detection of PI3K and AKT protein levels was not statistically significant. Conclusion: KIF3C may promote the proliferation and migration ability of lung cancer cells A549 through PI3K/AKT signaling pathway.
References
Nooreldeen R, Bach H, 2021, Current and Future Development in Lung Cancer Diagnosis. Int J Mol Sci, 22(16): 8661.
Jiang X, Guan J, Xu Y, et al., 2021, Silencing of CASC8 Inhibits Non-Small Cell Lung Cancer Cells Function and Promotes Sensitivity to Osimertinib via FOXM1. J Cancer, 12(2): 387–396.
Hirokawa N, Takemura R, 2004, Kinesin Superfamily Proteins and Their Various Functions and Dynamics. Exp Cell Res, 301: 50–59.
Son HJ, Choi EJ, Yoo NJ, et al., 2019, Somatic Frameshift Mutations of Cancer-Related Genes KIF3C and BARD1 in Colorectal Cancers. Pathol Res Pract, 215(10): 152579.
De S, Cipriano R, Jackson MW, et al., 2009, Overexpression of Kinesins Mediates Docetaxel Resistance in Breast Cancer Cells. Cancer Res, 69(20): 8035–8042.
Gao Y, Li L, Zheng H, et al., 2020, KIF3C is Associated with Favorable Prognosis in Glioma Patients and May be Regulated by PI3K/AKT/mTOR Pathway. J Neurooncol, 146(3): 513–521.
Imyanitov EN, Iyevleva AG, Levchenko EN, 2021, Molecular Testing and Targeted Therapy for Non-Small Cell Lung Cancer: Current Status and Perspectives. Crit Rev Oncol Hematol, 157: 103194.
Wang M, Herbst RS, Boshoff C, 2021, Toward Personalized Treatment Approaches for Non-Small-Cell Lung Cancer. Nat Med, 27(8): 1345–1356.
Hirokawa N, Noda Y, 2008, Intracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics. Physiol Rev, 88(3): 1089–1118.
Marszalek JR, Liu X, Roberts EA, et al., 2000, Genetic Evidence for Selective Transport of Opsin and Arrestin by Kinesin-II in Mammalian Photoreceptors. Cell, 102(2): 175–187.
Liu Z, Rebowe RE, Wang Z, et al., 2014, KIF3a Promotes Proliferation and Invasion Via Wnt Signaling in Advanced Prostate Cancer. Molecular Cancer Research, 12(4): 491–503.
Joseph NF, Grinman E, Swarnkar S, et al., 2020, Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical Neurons. Front Cell Neurosci, 14: 521199.
Huang X, Liu F, Zhu C, et al., 2014, Suppression of KIF3B Expression Inhibits Human Hepatocellular Carcinoma Proliferation. Digestive Diseases and Sciences, 59(4): 795–806.
Wang H, Li S, Liu B, et al., 2022, KIF11: A Potential Prognostic Biomarker for Predicting Bone Metastasis Free Survival of Prostate Cancer. Oncology Letters, 24(3): 312.
Zhao F, Feng Y, Zhang X, et al., 2020, Kinesin Superfamily Member 18B (KIF18B) Promotes Cell Proliferation in Colon Adenocarcinoma. Cancer Management and research, 12: 12769–12778.
Liu Q, Zhang X, Tang H, et al., 2020, Bioinformatics Analysis Suggests the Combined Expression of AURKB and KIF18B Being an Important Event in the Development of Clear Cell Renal Cell Carcinoma. Pathol Oncol Res, 26: 1583–1594.
Sun Z, Pan F, Shao J, et al., 2020, Kinesin Superfamily Protein 21B Acts as an Oncogene in Non-Small Cell Lung Cancer. Cancer Cell International, 20: 233.
Wang C, Wang C, Wei Z, et al., 2015, Suppression of Motor Protein KIF3C Expression Inhibits Tumor Growth and Metastasis in Breast Cancer by Inhibiting TGF-? Signaling. Cancer Lett, 368(1): 105–114.
Ma H, Zhang F, Zhong Q, et al., 2021, METTL3-Mediated m6A Modification of KIF3C-mRNA Promotes Prostate Cancer Progression and Is Negatively Regulated by miR-320d. Aging (Albany NY), 13(18): 22332–22344.
Liu H, Liu R, Hao M, et al., 2021, Kinesin Family Member 3C (KIF3C) Is a Novel Non-Small Cell Lung Cancer (NSCLC) Oncogene Whose Expression Is Modulated by microRNA-150-5p (miR-150-5p) and microRNA-186-3p (miR-186-3p). Bioengineered, 12(1): 3077–3088.
Xu L, Zhang X, Wang Z, et al., 2021, Kinesin Family Member 2A Promotes Cancer Cell Viability, Mobility, Stemness, and Chemoresistance to Cisplatin by Activating the PI3K/AKT/VEGF Signaling Pathway in Non-Small Cell Lung Cancer. Am J Transl Res, 13(4): 2060–2076.