Objective: To investigate the effect of small nucleolar host gene 12 (SNHG12) on the migration and invasion of human lung cancer cells by regulating human angiostatin binding protein through microribonucleic acid (microRNA)-497. Methods: A549, H1299, and PC9 cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium containing 10% fetal bovine serum, and human bronchial epithelial (HBE) cells were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum. The incubator conditions were as follows: saturated humidity, 37?, and 5% carbon dioxide (CO2). Results: The gene expressions of small nucleolar host gene 12 (SNHG12) in HBE, A549, H1299, and PC9 were 1.00 ± 0.02, 5.61 ± 0.42, 3.78 ± 0.29, and 3.51 ± 0.23, respectively. The gene expressions of microRNA-497HBE, A549, H1299, and PC9 were 1.00 ± 0.13, 0.21 ± 0.04, 0.35 ± 0.05, and 0.37 ± 0.06, respectively, with P < 0.05. The microRNA-497 gene expression and cell apoptosis rate in the microRNA-497 group and the microRNA-497 + pcDNA3.1 group were significantly higher than those in the miR-NC group, whereas the A value and cell invasion number were significantly lower than those in the miR-negative control (NC) group, with P < 0.05. Compared with the microRNA-497 + pcDNA3.1 group, the microRNA-497 gene expression and cell apoptosis rate in the microRNA-497 + SNHG12 group were significantly lower, whereas the A value and cell invasion number were significantly higher, with P < 0.05. Conclusion: SNHG12 can inhibit the migration and invasion of human lung cancer cells by regulating human angiostatin binding protein through microRNA-497.
Liang QS, Jia J, Hu X, Mir-519a-3p Regulates the Migration, Invasion and Apoptosis of Lung Cancer Cells by Targeting GALNT4 Gene. Hebei Pharmaceutical Sciences, 2022, 44(14): 2090–2095.
Duan Y, Yu J, Xu Y, et al., Expression of MAP3K9 in Lung Cancer and Its Effect on Proliferation, Migration and Invasion of Lung Cancer Cells. Modern Cancer Medicine, 2022, 30(15): 2685–2690.
Yao F, Shi W, Li C, et al., Microrna-223-3p Targeting TGFBR3 Affects Epithelial-Mesenchymal Transition and Wnt/?-Catenin Pathway in Lung Cancer Cells. Modern Cancer Medicine, 2022, 30(15): 2673–2679.
Liu B, Hu J, Hu Y, RNAcircSATB2 Regulates the Proliferation, Migration and Invasion of Lung Cancer Cells by Mir-382-5p. Journal of Clinical Pulmonology, 2022, 27(07): 1080–1085.
Zhang Q, Tong L, Ma H, Serum Exosomal Mir-21 Targeting PTEN Activates PI3K/AKT Signaling Pathway and Induces the Growth and Metastasis of Lung Cancer Cells. International Journal of Laboratory Medicine, 2022, 43(12): 1479–1484 + 1490.
Liu HM, Liu P, Liu HM, et al., LncRNALSINCT5 Regulates Invasion, Migration and Cisplatin Sensitivity of Lung Cancer Cells through Mir-451. Chin J Immunology, 2022, 38(11): 1349–1354.
Chen Z, Fang M, Xue H, et al., Deubiquitination Enzyme USP35 Promotes the Migration and Invasion of Non-Small Cell Lung Cancer Cells. Journal of Shandong University (Medical Edition), 2022, 60(04): 30–37.
Jiang S, Wang H, Guo H, et al., Effects of Long Non-Coding RNASNHG12 Targeting Mir-199a on Proliferation, Invasion and Metastasis of Tumor Cells. Chin J Experimental Surgery, 2022, 39(02): 336–339.
Zhang N, Shen H, Huang S, et al., 2021, The Role of LncRNASNHG12 in Promoting Hepatocellular Carcinoma Progression through Mir-129-5p/GTPBP4. Journal of the Third Military Medical University, 43(18): 1783–1795.
Zhou J, Liu J, Amidullah N, et al., 2021, Effects of Up-Regulation of LncRNA SNHG12 And Mir-199a-5p/FZD6 Axis on the Proliferation, Invasion and Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma Cells. Chin J General Surgery, 30(02): 173–186.
Yu H, 2021, Long Non-Coding RNASNHG12 Regulates Mir-30a-3p/RUNX2 Signaling Pathway to Promote Proliferation and Migration of Renal Clear Cell Carcinoma Cells, China Medical University.
Ma Q, Wei Y, Fu R, et al., 2019, Expression and Significance of LncRNA SNHG12, SNHG15 and SNHG16 in Lung Adenocarcinoma. Journal of Diagnostic Pathology, 26(05): 278–282 + 296.
Wang X, 2019, Meta-Analysis of Long Non-Coding RNA SNHG12 and Its Correlation with Tumor. Abstract Collection of the 14th Annual Meeting of Neurosurgeons of Chinese Medical Doctor Association, 914–915.
Liu X, 2019, Mechanism of PIWIL3/OIP5-AS1/Mir-367-3p/CEBPA and TDP43/SNHG12/Mir-195/SOX5 Feedback Loop Regulating Biological Behavior of Glioma Cells, China Medical University.