The The Effect of Elution Volume for Immunoprecipitation on m6A-Seq Analysis
Articles

The The Effect of Elution Volume for Immunoprecipitation on m6A-Seq Analysis

Yuhui Xu, Lize Shen, Guolin Li
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Keywords

m6A-Seq
Immunoprecipitation
Elution buffer

DOI

10.26689/par.v5i4.2239

Submitted : 2021-06-30
Accepted : 2021-07-15
Published : 2021-07-30

Abstract

Objective: To develop a cost-effective method to reduce the time consumption of elution in immunoprecipitation. Method: Two volumes (125 ?L for Group C and 100 ?L for Group T) of elution buffer were used to explore whether smaller volume could save testing time. Result: Time consumption of elution in Group T was significantly shorter than that in Group C, while the efficiency of eluted m6A-containing fragments and the performance of m6A-Seq as indicated by m6A peak distributions showed no difference between the two groups. Conclusion: A smaller volume of elution buffer was an economical way to reduce time consumption in immunoprecipitation.

References

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