Study on the Preparation of Doxorubicin Hydrochloride Liposomes and Their Therapeutic Effect on Liver Cancer

Abstract

Objective: This study aimed to prepare doxorubicin hydrochloride liposomes and explore their application value in patients with liver cancer. Methods: Doxorubicin hydrochloride liposomes were prepared using the ammonium sulfate gradient method. Doxorubicin, as a broad-spectrum antitumor drug, has significant toxic and side effects after toxicological investigation. After preparing DOX-Lip, single-factor analysis was used to analyze the effects of solution pH, number of ultrafiltration, oil-water ratio, incubation temperature, and time on the encapsulation efficiency of doxorubicin hydrochloride liposomes. The process was optimized through orthogonal experiments and then applied clinically. 110 patients with liver cancer were selected as the research subjects to verify the drug’s effectiveness. Results: The results of this study showed that under optimal process conditions, the prepared doxorubicin hydrochloride liposomes were evenly distributed, similar to spherical shapes, with an average particle size of 85–87 mm and a Zeta potential of 15–16 mV, indicating good encapsulation efficiency. The application of these liposomes to clinical treatment of liver cancer demonstrated good therapeutic effects and could effectively promote favorable patient prognosis. Conclusion: The doxorubicin hydrochloride liposomes prepared through process optimization exhibit strong stability and pronounced sustained-release characteristics, providing a solid foundation for the treatment of liver cancer.