Keywords
Multiply myeloma
HSP70
GEO database
Submitted : 2025-05-06
Accepted : 2025-05-21
Published : 2025-06-05
Abstract
Objective: This study aimed to investigate the changes in gene expression profiles of multiple myeloma (MM) cells after bortezomib treatment by analyzing the GEO database, thereby providing a theoretical foundation for subsequent research on HSP70. Methods: The GSE41929 dataset was selected from the GEO database. Screening and analysis were performed to identify differentially expressed genes between bortezomib-treated and non-treated MM cells. Results: After bortezomib treatment, 126 genes in MM cells showed the most significant changes in expression (P < 0.05, absolute value of logFC ≥ 1.5). Based on the fold change and the most significant gene module, HSPA1B exhibited the most notable upregulation after HMOX1, followed by HSPA6 and DNAJB1. HSPA1B and HSPA6 are members of the HSP70 protein family, while DNAJB1 primarily interacts with HSP70 to stimulate its ATPase activity and negatively regulates the transcriptional activity of HSF1 induced by heat shock. Conclusion: HSP70 was the most significantly upregulated molecule in MM cells following bortezomib stimulation.
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