Keywords
Interference
Biochemical detection
Submitted : 2023-02-27
Accepted : 2023-03-14
Published : 2023-03-29
Abstract
Objective: To explore the interference of monoclonal immunoglobulin (M protein) on the detection of serum LDL-C in patients with multiple myeloma, improve the understanding of this matter, determine and establish the correct method, and provide more accurate clinical results through this case. Methods: A case was selected for analysis by the direct method. Results: The interference of IgG kappa-type M protein on LDL-C detection could not be completely eliminated by the enzymatic method. Conclusion: IgG-type type M protein affects the detection of LDL-C by the enzymatic method; thus, light reagents can be used with the direct method for detection.
References
Zhang Y, Liu F, Tian H, et al., 2021, Clinical Significance of M Protein Concentration Detection in Patients with Monoclonal Immunoglobulinemia. Journal of Nephrology and Dialysis Kidney Transplantation, 30(2): 130–35.
Zhuang Y, 2020, Observation on the Effect of Hemolysis and Lipidemia in Serum Samples on the Results of Biochemical Tests. Clinical Research, 18(2): 112–113.
He J, Wang Z, Hu Y, 2013, Interference of IgM M Protein on Clinical Biochemical Detection. Chinese Medicine Guide, 11(15): 432–434.
Li J, Wang L, Zhao X, et al., 2011, Interference and Analysis of Rare IgA M Protein to Clinical Chemical Detection. Marker Immunoassay and Clinical Analysis, 18(6): 398–402.
King RI, Florkowski CM, 2010, How Paraproteins Can Affect Laboratory Assays: Spurious Results and Biological Effects. Pathology, 42: 397–401.
Yang Y, Howanitz PJ, Howanitz JH, et al., 2008, Paraproteins Are a Common Cause of Interferences with Automated Chemistry Methods. Archives of Pathology & Laboratory Medicine, 132(2): 217–223.