Mechanism of SNHG12 in Regulating Human Angiostatin Binding Protein Through MicroRNA-497 in the Migration and Invasion of Human Lung Cancer Cells
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Keywords

SNHG12
MicroRNA-497
Lung cancer
Cell migration and invasion

DOI

10.26689/par.v6i5.4336

Abstract

Objective: To investigate the effect of small nucleolar host gene 12 (SNHG12) on the migration and invasion of human lung cancer cells by regulating human angiostatin binding protein through microribonucleic acid (microRNA)-497. Methods: A549, H1299, and PC9 cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium containing 10% fetal bovine serum, and human bronchial epithelial (HBE) cells were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum. The incubator conditions were as follows: saturated humidity, 37?, and 5% carbon dioxide (CO2). Results: The gene expressions of small nucleolar host gene 12 (SNHG12) in HBE, A549, H1299, and PC9 were 1.00 ± 0.02, 5.61 ± 0.42, 3.78 ± 0.29, and 3.51 ± 0.23, respectively. The gene expressions of microRNA-497HBE, A549, H1299, and PC9 were 1.00 ± 0.13, 0.21 ± 0.04, 0.35 ± 0.05, and 0.37 ± 0.06, respectively, with P < 0.05. The microRNA-497 gene expression and cell apoptosis rate in the microRNA-497 group and the microRNA-497 + pcDNA3.1 group were significantly higher than those in the miR-NC group, whereas the A value and cell invasion number were significantly lower than those in the miR-negative control (NC) group, with P < 0.05. Compared with the microRNA-497 + pcDNA3.1 group, the microRNA-497 gene expression and cell apoptosis rate in the microRNA-497 + SNHG12 group were significantly lower, whereas the A value and cell invasion number were significantly higher, with P < 0.05. Conclusion: SNHG12 can inhibit the migration and invasion of human lung cancer cells by regulating human angiostatin binding protein through microRNA-497.

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