Effects of Different Doses of BDNF on Postoperative Cognitive Function in Aged Rats Undergoing Abdominal Surgery
Download PDF

Keywords

BDNF
Abdominal surgery
Aged rats
Postoperative cognitive function

DOI

10.26689/par.v6i4.4224

Submitted : 2022-06-28
Accepted : 2022-07-13
Published : 2022-07-28

Abstract

Objective: To investigate the effects of different doses of BDNF on postoperative cognitive function in aged rats undergoing abdominal surgery. Methods: 72 aged healthy male SD rats of SPF grade were selected. According to the random number table method, the rats were randomly divided into the control group, model group, low dose BDNF injection group, and high dose BDNF injection group, with 18 rats in each group. The model group, low dose group, and high dose group underwent abdominal surgery after anesthesia, and 5?L/time of BDNF was intranasally administered to the rats in the low dose and high dose groups 6 hours after abdominal surgery, of which the dose of the low dose group was 0.1 g/L, while that of the high dose group was 0.2 g/L. The drug was administered alternately through both nostrils, with an interval of 2 minutes each time, for 5 times. The control group did not undergo surgery after anesthesia. The escape latency and swimming distance of the four groups of rats were compared before surgery, the first day, the third day, and the seventh day after surgery; similarly, the BDNF protein expression level in the hippocampus of the four groups of rats was compared on the first day, the third day, and the seventh day after surgery. Results: The escape latency and swimming distance of the control group were not statistically significant on the first day, the third day, and the seventh day after surgery, p > 0.05; the escape latency and swimming distance of the model group, low dose group, and high dose group on the first day, the third day, and the seventh day after surgery were statistically significant, p < 0.05. Before surgery, the escape latency and swimming distance of the four groups were not statistically significant, p > 0.05; on the first day, the third day, and the seventh day after surgery, the escape latency and swimming distance of the model group > low dose group > high dose group > control group, p < 0.05. The BDNF protein expression level in the hippocampus of the control group on the first day, the third day, and the seventh day after surgery showed no statistical significance p > 0.05; the expression level of BDNF protein in the hippocampus of the model group, low dose group, and high dose group on the first day, the third day, and the seventh day after surgery was statistically significant, p < 0.05. On the first day, the third day, and the seventh day after surgery, the expression level of BDNF protein in the hippocampus of the model group < low dose group < high dose group < control group, p < 0.05. Conclusion: Compared with 0.1 g/L of BDNF, 0.2 g/L of BDNF can improve the postoperative cognitive function of aged rats undergoing abdominal surgery.

References

Wu X, Zhou Z, Wang H, et al., 2021, Feasibility of Cerebral Blood Flow / Cerebral Oxygen Metabolic Rate in Predicting Postoperative Cognitive Dysfunction in Elderly Patients Undergoing Non Cardiac Surgery. Zhejiang Medical Journal, 43(7): 716–720.

Shi J, He P, Zhao C, et al., 2021, Sedative Effect of Dexmedetomidine Combined with Spinal Anesthesia in Elderly Patients and Analysis of Risk Factors Affecting Postoperative Cognitive Dysfunction. International Journal of Geriatrics, 42(3): 158–162.

Liu D, Wu X, Pu G, et al., 2021, Construction and Validation of a Predictive Model for Postoperative Cognitive Impairment in Elderly Patients Undergoing Abdominal Surgery Under Non General Anesthesia. Nursing Research, 35(21): 3780–3784.

Cai S, Ye L, Zhang P, et al., 2021, Effects of Different Anesthesia Methods on Postoperative Cognitive Function and Peripheral Blood High Mobility Group Protein B1 in Elderly Patients Undergoing Gastrointestinal Surgery. Journal of Practical Clinical Medicine, 25(4): 41–44.

Chen Z, Li Z, Li S, 2021, Effects of Dexmedetomidine on Postoperative Cognitive Function, Brain Oxygen Metabolism and Delirium in Elderly Patients with Hip Fracture Surgery. Hainan Medical Journal, 32(21): 2762–2765.

Turkkahraman D, Sirazi EC, Aykal G, 2022, Serum Alpha-Melanocyte-Stimulating Hormone (A-MSH), Brain-Derived Neurotrophic Factor (BDNF), and Agouti-Related Protein (AGRP) Levels in Children with Prader-Willi or Bardet-Biedl Syndromes. Journal of Endocrinological Investigation, 45(5): 1031–1037.

Guo M, Dong L, Liu X, et al., 2021, Effect of Dexmedetomidine on the Level of Brain-Derived Nerve Growth Factor in Plasma of Patients with Meningioma After Operation. Stroke and Neurological Diseases, 28(4): 425–430.

Yang K, Hu G, Wang Y, et al., 2021, Effect of Acupuncture Point Pressing Method on Brain-Derived Nerve Growth Factor in Cerebral Cortex of Rats After Ischemic Stroke. Jilin Traditional Chinese Medicine, 41(6): 701–704.

Huang Y, Chen S, Huang J, 2021, TLR4/NF-? Role of B Signal Pathway in Sevoflurane Induced Postoperative Cognitive Dysfunction in Elderly Rats. Chinese Journal of Gerontology, 41(13): 2782–2785.

Zhao G, Shi J, Cheng J, et al., 2021, The Ameliorative Effect of Tangerine Peel on Learning and Memory Impairment in Aged Rats with Cognitive Impairment After Anesthesia. Journal of Northern Sichuan Medical College, 36(6): 689–695.

Fang K, Lu G, Wang X, et al., 2021, Effects of Exercise Training on Learning and Memory Impairment and Hippocampal Histopathological Changes in Mice with Mild Cognitive Impairment. PLA Journal of Medicine, 33(2): 5–9.

Wan H, Tao F, Zhao J, et al., 2021, Analysis of Influencing Factors of Postoperative Cognitive Dysfunction in Elderly Patients with Spinal Tuberculosis. Chinese Journal of Tuberculosis, 43(5): 441–445.

Zhang H, Guo X, Bai X, et al., 2021, Effects of Jianpi Zhidong Decoction on Cognitive Function and Brain-Derived Neurotrophic Factor in Rats with Chronic Stress-Induced Tic. Chinese Journal of Traditional Chinese Medicine, 36(8): 4676–4680.

Li Y, Si X, Niu J, et al., 2021, Research Progress of Brain-Derived Neurotrophic Factor and Its Tyrosine Kinase Receptor B Signaling Pathway in Neurological Diseases. Chinese Journal of Clinical Pharmacology, 37(9): 1121–1125.

Li J, Wang Y, Wen Y, et al., 2022, The Effect of Serum Cysc, BDNF and Vilip-1 Levels on Swallowing Dysfunction in Elderly Patients with Mild Cognitive Impairment and the Predictive Efficacy of Clinical Outcomes. Shandong Medicine, 62(14): 71–74.

Xing W, Zhang J, Wu X, et al., 2022, Research Progress on the Role of Brain-Derived Neurotrophic Factor and Neuroinflammation in Neurodegenerative Diseases. Chinese Medical Science, 12(10): 21–24.

Wang W, Zhuo Y, Hou Y, et al., 2022, Effects of Donepezil Hydrochloride Combined with Comprehensive Rehabilitation Training on Cognitive Function, Event-Related Potential and Serum BDNF and IGF-1 in Patients with Senile Dementia. Progress in Modern Biomedicine, 22(1): 99–102 + 112.

Chen X, Wang Z, 2021, Effects of Dexmedetomidine on Postoperative Cognitive Function and Plasma Mir-206 and BDNF Levels in Elderly Patients with Colorectal Cancer. Journal of Southeast University: Medical Edition, 40(4): 442–449.