Objective To explore the influence of different concentrations ofÂ isorhamnetin on C6 glioma cell morphology. Methods Set theÂ blank control group, blank solvent control group and reagent groupÂ of four concentration, the growth of cells were observed underÂ microscope; MTT assay was used to test the effect of isorhamnetinÂ on cultured C6 glioma cells, as well as calculate the cell inhibitionÂ rate and survival rate; flow cytometry was used to check theÂ detection peak and detection rate of Isorhamnetin group andÂ negative control apoptosis group, and analyzed the relationshipÂ between different concentrations of isorhamnetin and C6 gliomaÂ cell apoptosis rate; total protein was extracted from cells, and usedÂ Western blotting to detected total AKT protein and Ser473 AKTÂ protein loci in cells; used SD rats to construct brain glioma model,Â feed isorhamnetin plain to them for five days, and then used HPLCÂ to detect plasma, liver, brain tissue content. Results Under theÂ observation of inverted microscope and image analysis, after usingÂ Isorhamnetin, tumor cells appear apoptosis and necrosis change.Â Display with different Isorhamnetin MTT colorimetric methodÂ shows that the higher the concentration of added Isorhamnetin, theÂ worse the growth rate of C6 glioma cells in vitro, and the higherÂ the Inhibitory rate, the lower survival rate. The flow cytometricÂ detection shows the C6 glioma cells which is added 40 ug/ul Isorhamnetin have the highest rate of apoptosis. After adding 80 Î¼ g/ Î¼ l concentration of the isorhamnetin, C6 glioma cells have the lowest survival rate. Western blot test shows the AKT protein and Ser473 total site AKT protein density is in reverse proportion to the increase of the concentration of the isorhamnetin. High performance liquid chromatographic method has determined that there are isorhamnetin in both the rat plasma and brain tissue, which shows that the plasma and tissue all have different isorhamnetin Â distribution, Â and Â isorhamnetin mainly exist in the brain tissue. Conclusion Low concentration Â of Â isorhamnetin Â can Â induce apoptosis Â of Â C6 Â glioma Â cells, Â and Â high concentration of isorhamnetin can lead to apoptosis and necrosis of C6 glioma cells in vitro, which has obvious inhibitory effect on the growth of glioma cells, and the mechanism is closely related to PI3K/AKT pathway, and in SD rat brain glioma model , the high performance liquid chromatography was used to detect the content of plasma and brain tissue, which indicated the isorhamnetin has target in brain tissue, which provided Â experimental Â evidence Â for Â the development and utilization of isorhamnetin in mice.