Experimental Research on Intratumoral Injection of Sal Ammoniac Extract in the Treatment of Mouse Liver Cancer

Abstract

Objective: To study the effect of sal ammoniac extract on the treatment of liver cancer and analyze its possibility of replacing absolute ethanol. Methods: Sixty Kunming mice (5-6 weeks old, 18-22g in weight, male and female in half) were selected and inoculated with 0.1 ml of 1:4 ascitic diluent from mouse liver cancer H22 under the axilla of the right limb. After tumor formation, they were randomly divided into 3 groups with 20 mice in each group. Normal saline (NS), sal ammoniac extract (N), and absolute ethanol (E) were injected into the tumor once a day for four times. The death, tumor weight, tumor inhibition rate, lactate dehydrogenase (LDH), and succinate dehydrogenase (SDH) expression of the mice in each group were analyzed. Results: In the course of treatment, 3 mice died in the normal saline group, 2 mice died in the sal ammoniac treatment group, and 7 mice died in the absolute ethanol group. The tumor weight of the normal saline group, sal ammoniac treatment group, and absolute ethanol group were 1.88 ± 0.26, 1.41 ± 0.49, and 1.51 ± 0.46, respectively. The tumor inhibition rates were 0%, 31.0%, and 21.6%, respectively. Comparing the sal ammoniac treatment group and the normal saline group, t = 3.5133, p = 0.0013; comparing the absolute ethanol group and the normal saline group, t = 2.7926, p = 0.0093. The expression of LDH was 81 ± 10, 51 ± 11, and 32 ± 9 in the normal saline group, sal ammoniac treatment group, and absolute ethanol group, respectively, while the expression of SDH was 80 ± 10, 51 ± 10, and 51 ± 12 in the normal saline group, sal ammoniac treatment group, and absolute ethanol group, respectively. Comparing the LDH of the sal ammoniac treatment group with that of the saline group, t = 8.4264, p = 0.0000; comparing the absolute ethanol group and normal saline group, t = 13.8763, p = 0.0000. Comparing the SDH of the sal ammoniac treatment group with that of the normal saline group, t = 8.1455, p = 0.0000; comparing the absolute ethanol group with the normal saline group, t = 7.2197, p = 0.0000. Conclusion: The traditional Chinese antitumor medicine, sal ammoniac and its main effective components have good antitumor effect, which can be further popularized and applied in clinical practice.