Abstract
Objectives: Bone formation and bone resorption continuously
occur in bone tissue to prevent the accumulation of old bone, this
being called bone remodeling. Osteoblasts especially play a crucial
role in bone formation through the differentiation and proliferation.
Therefore, in this study, we investigated the effects of collagen
hydrolysate Glycine-Prolyl-Hydroxyproline (Gly-Pro-Hyp) on
osteoblastic proliferation and differentiation in MC3T3-E1 cells.
Methods: Four groups including control, Gly-Pro-Hyp 20 μM,
100 μM, 500 μM groups were set up in this study. Cells were
cultured with blank control medium or Gly-Pro-Hyp of the
different dosages for 24 h. CCK 8 assay was analyzed cell
proliferation. Assay of Alkaline phosphatase (ALP)activity was
analyzed osteoblast differentiation. The expression levels of ALP,
Col 1, Runx 2 and Osterix in MC3T3-E1 cells were measured by
Western blot. Results: The results indicated the treatment of Gly-
Pro-Hyp promoted the proliferation of MC3T3-E1 cells and
improved ALP activity. In addition, cells treated with Gly-Pro-Hyp
significantly upregulated protein expression of ALP, type 1
collagen, runt-related transcription factor 2 and osterix.
Conclusion: The results demonstrate that Gly-Pro-Hyp promote
differentiation inducement and proliferation of MC3T3-E1 cells,
therefore may help to elucidate the transcriptional mechanism of
bone formation and possibly lead to the development of bone-
forming drugs.