Abstract
Objective: investigate that the transplantation of sonic hedgehog
(SHH) fibrin scaffold promotes recovery of the spinal cord injury
in rats. Method: first, the model of controlled-release SHH fibrin
scaffold was made in vitro as the experimental group and observe
the controlled-release performance. Second, 60 healthy SD rats
were assigned to prepare models of complete transection of spinal
cord, divided into 3 groups: SCI group (simple transection of
spinal cord), FG group (fibrin group), F-SHH group (sonic
hedgehog-fibrin scaffold transplantation group). Grade hind limbs
(BBB) of rats every week. The spinal cord segments were got out
in 3 months after operation and went through
immunohistochemistry and immunoblotting detection. Observe the
expression of NF200, GAP43 and GFAP. Result: (1) SHH Fibrin
showed a good effect of slow release. (2) F-SHH group showed a
more significant improvement in BBB score that presented a rising
trend in the whole, compared with the other two groups and the
difference is statistically significant (P < 0.05). (3) The relative
amounts of NF200 and GAP43 in F-SHH group were much higher
than those in the other two groups, while the relative amount of
GFAP was lower and the difference is statistically significant (P <
0.05). Conclusion: Controlled-release SHH fibrin scaffold
transplantation will effectively recover complete spinal cord
transection of rats.